For porcine IL-17A, the characterization of a panel of 10 mAbs identified eight different antigenic determinants interestingly, most of the mAbs cross-reacted with the dolphin recombinant ortholog. We screened each mAb for cross-species reactivity with orthologs of IL-17A or IFNγ and checked each mAb for inhibition by other related mAbs, to assign mAb antigenic determinants. Swine interleukin-17A (IL-17A) and Interferon-gamma (IFNγ) recombinant proteins were produced using yeast expression and used for monoclonal antibody (mAb) production resulting in panels of mAbs. Our efforts were aimed at the expression of soluble swine cytokines and the production of panels of monoclonal antibodies (mAbs) to these proteins. The goal of this study was to generate priority immune reagents for pigs and pipeline them for marketing. 4Bristol Veterinary School, University of Bristol, Bristol, United KingdomĬurrent research efforts require a broad range of immune reagents, but those available for pigs are limited.2Center for Food Animal Health, Department of Animal Sciences, The Ohio State University, Wooster, OH, United States.1Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center (BARC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), Beltsville, MD, United States.Renukaradhya 2, Joanna LaBresh 3, Yvonne Sullivan 3, Ore Francis 4 and Joan K. Walker 1, Veerupaxagouda Patil 2, Gourapura J.
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